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[DOI] [PMC free article] [PubMed] [Google Scholar] 210.Assimakopoulos S.F., Vagianos C.E., Zervoudakis G., Filos K.S., Georgiou C., Nikolopoulou V., Scopa C.D

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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at −80 °C up to processing