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In order to distinguish the exact cellular site where DDX3X and pUs3 interact, we fractionated our cells into cytoplasm, nuclear matrix and nuclear membranes prior to co-IP

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Results Metfromin-cytotoxicity is recovered by glucose available To understand biochemical pathways regulating metformin-induced cancer cell death, HeLa cells maintained in DMEM with 5.5 mM glucose were treated with metformin (5 mM) along with daily addition of glucose (0–2 mM) for 72 h