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During autooxidation of glucose, enzymatic pathways, such as the NOX pathway, become the main sources of ROS 38,39

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A critical 2017 review by Giustarini and colleagues in Antioxidants and Redox Signaling documents the technical challenges: plasma GSH is present at very low concentrations and is highly susceptible to pre-analytical degradation from improper blood handling, temperature changes, and sample processing delays